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thermal cycler dice real time pcr system 18  (TaKaRa)


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    Structured Review

    TaKaRa thermal cycler dice real time pcr system 18
    Thermal Cycler Dice Real Time Pcr System 18, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 7997 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr+thermal+cycler+dice+system/Thermal+Cycler+Dice+Real+Time+System/pm42017305-81-5-12
    Average 98 stars, based on 7997 article reviews
    thermal cycler dice real time pcr system 18 - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Amplification:

    Article Title: Characterization of the Non-rhizobial Bacterial Community in the Nodule-Associated Soils of Akebono Soybeans and Isolation of Antibiotic Producing Amycolatopsis spp.
    Article Snippet: The unique Akebono soybeans are cultivated in Minobu Town, Yamanashi Prefecture, Japan.. The biogeography of Akebono soybeans and the microbial diversity associated with their root nodules remain unexplored.. This study investigated the nodule-associated microbial community of Akebono soybeans using molecular techniques.

    Article Title: Detection of MEAF6-PHF1 translocation in an endometrial stromal nodule.
    Article Snippet: Endometrial stromal nodule (ESN) and low-grade endometrial stromal sarcoma (LG-ESS) are rare uterine tumors known as endometrial stromal tumors (ESTs).. In addition to their similarity in morphological features, recent studies have shown that these two tumors share common genetic alterations.. In particular, JAZF1-SUZ12 fusion is found with high frequency in both ESN and LG-ESS.

    Article Title: Genetic characterization of the male-sterile Japanese cedar ‘Fukushima-funen 3’ reveals a naturally pyramided genotype and a novel MS2 allele, and leads to the development of marker-assisted selection for the MS2 locus
    Article Snippet: PCR was performed in a 15-μL reaction volume containing approximately 5 ng of genomic DNA, 1 × KAPA2G buffer with 1.5 mM MgCl2, 0.2 mM of each dNTP, 0.5 mM MgCl2, 0.2 μM each of forward and reverse primers (Table 2a), and 0.5 U KAPA2G fast DNA polymerase (KAPA2G Fast PCR Kit; KAPA Biosystems). .. Amplification was performed on a TaKaRa PCR Thermal Cycler Dice system (Takara Bio) under the following conditions: initial denaturation for 3 min at 95 ◦C, followed by 35 cycles of denaturation for 15 s at 95 ◦C, annealing for 15 s at 60 ◦C, extension for 1 s at 72 ◦C, and a final extension for 1 min at 72 ◦C. .. The PCR products were electrophoresed at 100 V for 25 min on 2 % agarose gel in tris-acetate-ethylenediaminetetraacetic acid buffer, and stained with Midori Green Extra (Nippon Genetics).

    Polymerase Chain Reaction:

    Article Title: Characterization of the Non-rhizobial Bacterial Community in the Nodule-Associated Soils of Akebono Soybeans and Isolation of Antibiotic Producing Amycolatopsis spp.
    Article Snippet: The unique Akebono soybeans are cultivated in Minobu Town, Yamanashi Prefecture, Japan.. The biogeography of Akebono soybeans and the microbial diversity associated with their root nodules remain unexplored.. This study investigated the nodule-associated microbial community of Akebono soybeans using molecular techniques.

    Article Title: Specialized Pro-Resolving Mediators Do Not Inhibit the Synthesis of Inflammatory Mediators Induced by Tumor Necrosis Factor-α in Synovial Fibroblasts.
    Article Snippet: Specif ic primers (Table 1) were purchased from Thermo Fisher Scientific. .. PCR was conducted using the TaKaRa PCR Thermal Cycler Dice system (Takara Bio, Kusatsu, Japan) under the following conditions: initial holding at 25°C for 10 min, 42°C for 60 min, and 85°C for 5 min. Real-time PCR was performed on a ViiA7 Real-Time PCR system (Thermo Fisher Scientific) for 40 cycles at 95°C for 1 s and 60°C for 20 s. The expression levels of COX-2, mPGES-1, IL6, and MMP-3 were normalized to glyceraldehyde 3- phosphate dehydrogenase (GAPDH). ..

    Article Title: Geranylgeraniol Inhibits Lipopolysaccharide-Induced Inflammation in Mouse-Derived MG6 Microglial Cells via NF-κB Signaling Modulation
    Article Snippet: .. Four micrograms of RNA were used to synthesize cDNA by denaturing them with oligo-dT primers and dNTPs at 65 °C for 5 min in a TaKaRa PCR Thermal Cycler Dice system (Takara Bio). ..

    Article Title: Dietary analysis of the House Swift (Apus nipalensis) in Hong Kong using prey DNA in faecal samples
    Article Snippet: .. The Takara PCR Thermal Cycler Dice system was used for all amplifications. ..

    Article Title: Detection of MEAF6-PHF1 translocation in an endometrial stromal nodule.
    Article Snippet: Endometrial stromal nodule (ESN) and low-grade endometrial stromal sarcoma (LG-ESS) are rare uterine tumors known as endometrial stromal tumors (ESTs).. In addition to their similarity in morphological features, recent studies have shown that these two tumors share common genetic alterations.. In particular, JAZF1-SUZ12 fusion is found with high frequency in both ESN and LG-ESS.

    Article Title: Genetic characterization of the male-sterile Japanese cedar ‘Fukushima-funen 3’ reveals a naturally pyramided genotype and a novel MS2 allele, and leads to the development of marker-assisted selection for the MS2 locus
    Article Snippet: PCR was performed in a 15-μL reaction volume containing approximately 5 ng of genomic DNA, 1 × KAPA2G buffer with 1.5 mM MgCl2, 0.2 mM of each dNTP, 0.5 mM MgCl2, 0.2 μM each of forward and reverse primers (Table 2a), and 0.5 U KAPA2G fast DNA polymerase (KAPA2G Fast PCR Kit; KAPA Biosystems). .. Amplification was performed on a TaKaRa PCR Thermal Cycler Dice system (Takara Bio) under the following conditions: initial denaturation for 3 min at 95 ◦C, followed by 35 cycles of denaturation for 15 s at 95 ◦C, annealing for 15 s at 60 ◦C, extension for 1 s at 72 ◦C, and a final extension for 1 min at 72 ◦C. .. The PCR products were electrophoresed at 100 V for 25 min on 2 % agarose gel in tris-acetate-ethylenediaminetetraacetic acid buffer, and stained with Midori Green Extra (Nippon Genetics).

    Article Title: Specialized Pro-Resolving Mediators Do Not Inhibit the Synthesis of Inflammatory Mediators Induced by Tumor Necrosis Factor-α in Synovial Fibroblasts
    Article Snippet: Specific primers ( ) were purchased from Thermo Fisher Scientific. .. PCR was conducted using the TaKaRa PCR Thermal Cycler Dice system (Takara Bio, Kusatsu, Japan) under the following conditions: initial holding at 25°C for 10 min, 42°C for 60 min, and 85°C for 5 min. Real-time PCR was performed on a ViiA7 Real-Time PCR system (Thermo Fisher Scientific) for 40 cycles at 95°C for 1 s and 60°C for 20 s. The expression levels of COX-2, mPGES-1, IL-6, and MMP-3 were normalized to glyceraldehyde 3- phosphate dehydrogenase (GAPDH). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Specialized Pro-Resolving Mediators Do Not Inhibit the Synthesis of Inflammatory Mediators Induced by Tumor Necrosis Factor-α in Synovial Fibroblasts.
    Article Snippet: Specif ic primers (Table 1) were purchased from Thermo Fisher Scientific. .. PCR was conducted using the TaKaRa PCR Thermal Cycler Dice system (Takara Bio, Kusatsu, Japan) under the following conditions: initial holding at 25°C for 10 min, 42°C for 60 min, and 85°C for 5 min. Real-time PCR was performed on a ViiA7 Real-Time PCR system (Thermo Fisher Scientific) for 40 cycles at 95°C for 1 s and 60°C for 20 s. The expression levels of COX-2, mPGES-1, IL6, and MMP-3 were normalized to glyceraldehyde 3- phosphate dehydrogenase (GAPDH). ..

    Article Title: Specialized Pro-Resolving Mediators Do Not Inhibit the Synthesis of Inflammatory Mediators Induced by Tumor Necrosis Factor-α in Synovial Fibroblasts
    Article Snippet: Specific primers ( ) were purchased from Thermo Fisher Scientific. .. PCR was conducted using the TaKaRa PCR Thermal Cycler Dice system (Takara Bio, Kusatsu, Japan) under the following conditions: initial holding at 25°C for 10 min, 42°C for 60 min, and 85°C for 5 min. Real-time PCR was performed on a ViiA7 Real-Time PCR system (Thermo Fisher Scientific) for 40 cycles at 95°C for 1 s and 60°C for 20 s. The expression levels of COX-2, mPGES-1, IL-6, and MMP-3 were normalized to glyceraldehyde 3- phosphate dehydrogenase (GAPDH). ..

    Expressing:

    Article Title: Specialized Pro-Resolving Mediators Do Not Inhibit the Synthesis of Inflammatory Mediators Induced by Tumor Necrosis Factor-α in Synovial Fibroblasts.
    Article Snippet: Specif ic primers (Table 1) were purchased from Thermo Fisher Scientific. .. PCR was conducted using the TaKaRa PCR Thermal Cycler Dice system (Takara Bio, Kusatsu, Japan) under the following conditions: initial holding at 25°C for 10 min, 42°C for 60 min, and 85°C for 5 min. Real-time PCR was performed on a ViiA7 Real-Time PCR system (Thermo Fisher Scientific) for 40 cycles at 95°C for 1 s and 60°C for 20 s. The expression levels of COX-2, mPGES-1, IL6, and MMP-3 were normalized to glyceraldehyde 3- phosphate dehydrogenase (GAPDH). ..

    Article Title: Specialized Pro-Resolving Mediators Do Not Inhibit the Synthesis of Inflammatory Mediators Induced by Tumor Necrosis Factor-α in Synovial Fibroblasts
    Article Snippet: Specific primers ( ) were purchased from Thermo Fisher Scientific. .. PCR was conducted using the TaKaRa PCR Thermal Cycler Dice system (Takara Bio, Kusatsu, Japan) under the following conditions: initial holding at 25°C for 10 min, 42°C for 60 min, and 85°C for 5 min. Real-time PCR was performed on a ViiA7 Real-Time PCR system (Thermo Fisher Scientific) for 40 cycles at 95°C for 1 s and 60°C for 20 s. The expression levels of COX-2, mPGES-1, IL-6, and MMP-3 were normalized to glyceraldehyde 3- phosphate dehydrogenase (GAPDH). ..



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